|Title||Research Associate III|
|Office Building||Animal Disease Research & Diagnostic Laboratory|
|Mailing Address||South Dakota State University
Veterinary & Biomedical Sciences
SAR 116, Box 2175
Brookings, SD 57007
Academic Interests/ExpertiseDiagnostic Test Development & Reagent (monoclonal antibody) Production
Molecular Biology & Molecular Diagnostics.
Transboundary & Emerging Diseases.
Area(s) of ResearchDr. Lawson’s research interests focus on advances in viral diagnostics for the swine production industry. Over the last 15 years, his research has involved characterizing molecular mechanisms of disease & viral persistence of porcine reproductive and respiratory syndrome virus (PRRSV). Currently, his research is focused on developing antibody reagents for improved viral diagnostics and developing molecular tools for investigating parameters of virus-host immunity. This includes developing new assays for porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and senecavirus A (SVA). Dr. Lawson’s laboratory brings expertise within areas of molecular biology including gene cloning, protein expression & purification, and the development of reverse genetic systems for research. He and colleagues have relied on the development of expressed antigen and monoclonal antibody production for use as diagnostic tools for the development of a wide range of new diagnostic testing platforms including IFA, ELISA and the Luminex-based fluorescent microsphere immunoassay (FMIA). These assays fill a diagnostic need for the swine industry to rapidly detect multiple pathogens and provide important tools for disease surveillance and testing of vaccine candidates for the prevention of disease.
Creative ActivitiesPEER REVIEWED PUBLICATIONS:
Joshi, L.R., Fernandes, M.H.V., Clement, T., Lawson, S., Pillatzki, A., Resende, T.P., Vanucci, F.A., Kutish, G.F., Nelson E.A., Diel, D.G. 2016. Pathogenesis of Senecavirus A infection in finishing pigs. Journal of General Virology. Oct 14 doi:10.1099/jgv.0.000631.
Diel, D.G., S. Lawson, F. Okda, A. Singrey, T. Clement, M. H. V. Fernandes, J. Christopher-Hennings, E. A. Nelson. 2016. Porcine epidemic diarrhea virus: an overview of current virological and serological diagnostic methods. Virus Research. doi:10.1016/j.virusres.2016.05.013.
Hain, K.S., L.R. Joshi, F. Okda, J. Nelson, A. Singrey, S. Lawson, A. Pillatzki, G.F. Kutish, E.A. Nelson, E.F. Flores, D.G. Diel. Immunogenicity of a Recombinant Parapoxvirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus. 2016. J. Gen. Virol. doi: 10.1099/jgv.0.000586.
Okda, F., *S. Lawson, X. Liu, A. Singrey, T. Clement, J. Nelson, J. Christopher-Hennings, E.A. Nelson. 2016. Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirus. BMC Vet. Res. 12:95. doi: 10.1186/s12917-016-0716-6.
Clement, T., A. Singrey, S. Lawson, F. Okda, J. Nelson, D. Diel, E.A. Nelson, J. Christopher-Hennings. 2016. Quantitation of porcine epidemic diarrhea virus neutralizing antibodies in sow serum, colostrum, milk and piglet serum after feedback. J. Swine Health and Production 24:147-153.
Okda, F., X. Liu, A. Singrey, T. Clement, J. Nelson, J. Christopher-Hennings, E.A. Nelson, *S. Lawson. 2015. Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus. BMC Vet. Res. 11:180. doi:10.1186/s12917-015-0500-z.
Jane Christopher-Hennings, Karla P. C. Araujo, Carlos J. H. Souza, Ying Fang, Steven Lawson, Eric A. Nelson, Travis Clement, Michael Dunn, Joan K. Lunney. 2013. Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories. Journal of Veterinary Diagnostic Investigation.
Li Y, Zhu L, Lawson SR, Fang Y. 2013. Targeted mutations in a highly conserved motif of the nsp1β protein impair the interferon antagonizing activity of porcine reproductive and respiratory syndrome virus. J Gen Virol. Sep;94(Pt 9):1972-83.
Sun Z, Lawson S, Langenhorst R, McCormick KL, Brunick C, Opriessnig T, Baker R, Yoon KJ, Zhang W, Huber VC, Fang Y. 2013. Construction and immunogenicity evaluation of an epitope-based antigen against swine influenza A virus using Escherichia coli heat-labile toxin B subunit as a carrier-adjuvant. Vet Microbiol. Jun 28;164(3-4):229-38. (Co-first author with Sun)
Lawson SR., Li Y., Patton J., Langenhorst RJ., Sun Z., Jiang Z., Christopher-Hennings J., Nelson EA., Knudsen D., Fang Y., Chang KO. 2012. Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus. Virus Res. Feb; 163(2): 461-8.
Langenhorst RJ, Lawson SR, Kittawornrat A, Zimmerman JJ, Sun Z, Li Y, Christopher-Hennings J, Nelson EA, Fang Y. 2012. Development of a fluorescent microsphere immunoassay for detection of antibodies against porcine reproductive and respiratory syndrome virus using oral fluid samples as an alternative to serum-based assays. Clin Vaccine Immunol. Feb; 19(2):180-9. (Co-first author with Langenhorst)
EducationPh.D. - Biological Science/Virology. South Dakota State University, Brookings, SD.
B.S. - Biological Science. University of California, Davis. Davis, California.