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Seneca Valley Virus in Swine:

In 2015, the incidence of Seneca Valley Virus has increased and clinical signs in pigs can be observed. The clinical signs are often very similar to other vesicular foreign animal disease symptoms. Therefore, if vesicular lesions are observed, the State Veterinarian's office should be contacted as the first step in diagnosis Dr. Dustin Oedekoven, (dustin.oedekoven@state.sd.us) Phone:605-773-3321, Fax: 605-773-5459) http://aib.sd.gov/ The South Dakota Animal Disease Research and Diagnostic Laboratory currently has a real-time PCR available for testing for this virus.

For more information on Seneca Valley Virus, please see the following web link from the Swine Health Information Center: http://www.swinehealth.org/emerging-disease-information/

Vesicular Stomatitis Virus

Vesicular Stomatitis in South Dakota - For testing horses for VSV at the laboratory:

  1. Serum (for CF testing )
  2. Swabs from the vesicular lesions (can be sent in TBTB media or viral transport media) for PCR testing.
  1. Contact the SD AIB (605) 773-3321
  2. Your veterinarian should collect BOTH:
  3. Send to the SD Animal Disease Research and Diagnostic Laboratory (SD ADRDL), (605) 688-5171, with the submission form (see http://www.sdstate.edu/vs/adrdl) and request VSV CF and VSV PCR
     

Canine Influenza Testing – SDSU ADRDL

NOTE: Timing of collection is critical to viral detection as canine influenza virus is only shed 2–4 days after initial clinical signs. Virus is not likely to be present in samples taken later in the course of disease, even if the dog is still showing clinical signs.

Swab: Polyester, Dacron, or rayon swab with a plastic shaft (BD BLL sterile culture swabs are OK)

Procedure:

  • Nasal swabs: insert swab into nares.
  • Pharyngeal swabs: swab the back of the throat,near the end of the soft palate.
  • Collect both nasal and deep pharyngeal swabs should be collected.
  • Place swab back into tube and add several drops of saline to keep the tube moist.
  • If you have parafilm, cover the tube with it to seal it.
  • Keep the samples refrigerated until they can be mailed (preferably the day of sampling).
  • Mail with ice packs and mail overnight.

Testing is performed daily at a cost of $40/sample. The test is a real-time PCR procedure that detects Influenza A. If subtyping to characterize the virus as the canine H3N2 strain is desired, we can forward the sample on for further testing.

To submit, go to http://www.sdstate.edu/vs/adrdl/index.cfm click on "submission and other forms" on the left side of the page to find the "all species health form." Write in "canine influenza testing" and include the form with the sample.