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New PCR test detects PRRS virus and Influenza A in the same sample

Testing for two important disease-causing germs in pigs is getting easier and more cost-effective, thanks to a new test brought online at the SDSU ADRDL.

A cost effective method for simultaneously detecting porcine reproductive and respiratory syndrome (PRRSV) and influenza A virus in swine (IAV-S) for respiratory disease surveillance was developed at Tetracore, Inc. (Rockville, MD) and validated here at the South Dakota Animal Disease Research and Diagnostic Laboratory. This assay was created by combining Tetracore’s EZ-PRRSV™ MPX 4.0 and EZ-Universal Flu 2.0 assays without modifications.

These individual real-time reverse transcription polymerase chain reaction (rtRT-PCR) tests for both PRRSV and IAV-S are well established and have been used at the ADRDL for routine diagnostic testing. The new multiplex PCR assay will reduce laboratory testing costs for the producer and promote the continued surveillance for both PRRSV and IV-S. Recently, the USD discontinued funding for IAV-S matrix (screening) testing making the cost of the IAV-S surveillance screening by rtRT-PCR the responsibility of the producer. Through the use of this new multiplex PCR test, the producer should see a 50% cost savings over using the two standalone tests for the detection of PRRSV and IV-S.

What does this mean for samples submitted to the diagnostic lab for PRRSV and/or IAV-S PCR testing? The newly developed test has been validated for use with oral fluids, nasal swabs, tissues, and oropharyngeal swabs. Starting on May 22, 2017, all oral fluids, nasal swabs, tissues, and oropharyngeal swabs submitted for PRRSV and/or IAV-S will be tested with the combined PRRSV/IAV-S multiplex assay. It is important to note that the PRRSV/IAV-S multiplex is not validated for use with serum or whole blood in saline (swab) samples. The newly multiplexed test for PRRSV/IAV-S does not differentiate between North American and European PRRSV strains but will detect all known North American and European PRRSV isolates.

The cost for PRRSV/IAV-S multiplex PCR is $25.00 and the test is run daily on samples that arrive before 11:00 AM. If you have any questions, please call the laboratory at (605) 688-5171 and ask to speak with the Molecular Diagnostics section.