We offer DNA sequencing services using Illumina NextSeq 500 and MiSeq platforms. We have capabilities to prepare several common libraries types. We can also perform automated DNA and RNA extractions as well as perform PCRs for amplicon sequencing project using a pipetting robot. We QC all samples and libraries.
We can help you with detail experimental design and bioinformatic analysis in a collaboration basis. If you are interested in that possibility make sure to discuss it with us.
This facility is funded through a Major Research Instrumentation grant from the National Science Foundation with support from the South Dakota Agricultural Experimental Station. Additionally, BioSNTR (a regional Biosciences virtual center funded thru the NSF-EPSCOR and the SD Governor’s Office for Economic Development) is making a significant investment in the facility.
Here is what researchers say about having these services available locally:
“Having a sequencing center in South Dakota allows researchers to rapidly acquire genomic data, conduct bioinformatics analyses and then refine their experimental designs,”
Adam Hoppe, director of BioSystems Networks and Translational Research Center
“The next-generation sequencing facility will increase the precision in gene transfers. It will help us develop genomic prediction models so that we can deliver better genetics and varieties to the producers of the South Dakota.”
SDSU winter wheat breeder Sunish Seghal
The availability of an in-house sequencing facility means shorter turn-around time in getting sequencing results.
Associate professor Senthil Subramanian of Agronomy, Horticulture and Plant Science