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Bruker BIFLEX IV MALDI-TOF

Picture of Bruker BIFLEX IV MALDI_TOF

About the MALDI-TOF

Specifications

  • Mass Range: m/z 500-1,000,000
  • Resolution (m/Δm): 5,000 (linear mode), 20,000 (reflectron mode)
  • Accuracy: 200 ppm (linear mode), 10 ppm (reflectron mode)
  • 337 nm Laser
  • Ionization Source: Matrix-assisted laser desorption ionization (MALDI)
  • Mass Analyzer: Time of flight (TOF)

Common Applications

  • Characterization of biopolymers (proteins, protein digests and oligonucleotides)
  • Organometallic complexes
  • Some synthetic polymers
  • Molecules that photoionize upon radiation by 337 nm laser
  • Labile molecules

Publications using a MALDI-TOF

  • Victor, L.; Fennell, A.; Grimplet, J. Proteomic analysis of shoot tissue during photoperiod induced growth cessation in V. riparia Michx. grapevines. Proteome Sci. 2010, 8.
  • Moussaoui, W.; Jaulhac, B.; Hoffmann, A.; Kostrzewa, M. Riegel, P.; Prevost, G. Matrix assisted laser desorption ionization time-of-flight mass spectrometry identifies 90% of bacteria directly from blood vials. 2010, 16 (11), 1631-1638.
  • Karamonova, L.; Junkova, P.; Mihalova, D.; Javurkova, B.; Fukal, L.; Rauch, P.; Blazkova, M. The potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of biogroups of Cronobacter sakazakii. Rapid Commun. Mass Spectrom.2013, 27 (3), 409-418.
  • Kim, Y.; Kim, S.; Chung, H.; Jee, M.; Kim, B.; Kim, K.; Yoon, J.; Lee, H.; Kim, C.; Kim, S; Yoo, W.; Hong, S. Population genotyping hepatitis C virus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of short DNA fragments. Clin. Chem. 2005, 51 (7), 1123-1131.
  • Bonk, T.; Humeny, A.; Gebert, J.; Sutter, C.; von Knebel Doeberitz, M.; Becker, C. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based detection of microsatellite instabilities in coding DNA sequences: A novel approach to identify DNA-mismatched repair-deficient cancer cells. Clin. Chem. 2003, 49 (4), 552-561.