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Canine Influenza Testing –SDSU ADRDL

NOTE: Timing of collection is critical to viral detectionas canine influenza virus is only shed 2-4 days after initial clinical signs. Virus is not likely to be present in samples takenlater in the course of disease, even if the dog is still showing clinical signs.

Swab: Polyester, Dacron, or rayon swab with a plasticshaft (BD BLL sterile culture swabs are OK)

Procedure:

·        Collect both nasal and deep pharyngeal swabsshould be collected.

o  Nasal swabs: insert swab into nares.

o  Pharyngeal swabs: swab the back of the throat,near the end of the soft palate.

·        Place swab back into tube and add several dropsof saline to keep the tube moist.

·        If you have parafilm, cover the tube with it toseal it.

·        Keep the samples refrigerated until they can bemailed (preferably the day of sampling).

·        Mail with ice packs and mail overnight.

Testing is performed daily at a cost of $40/sample.The test is a real-time PCR procedure thatdetects Influenza A.If subtyping tocharacterize the virus as the canine H3N2 strain is desired, we can forward thesample on for further testing.

To submit, go to http://www.sdstate.edu/vs/adrdl/index.cfm click on "submission and other forms" on theleft side of the page to find the "all species health form." Write in "canineinfluenza testing" and include the form with the sample.

Prompt and Accurate Veterinary Diagnostic Services

The South Dakota Animal Disease Research and Diagnostic Laboratory (ADRDL) has served the citizens of the state and region with timely and accurate veterinary diagnostic services since 1887. A dedicated and experienced staff performs a full range of diagnostic testing services that arm veterinarians and health officials with the information they need to protect and improve animal and therefore, human health.

microscopeThe staff of the ADRDL are nationally recognized for their skill in diagnosing key diseases of cattle, pigs, and other livestock. Additionally, the ADRDL supports veterinarians and caretakers of horses and companion animals, plays a vital role in identifying zoonotic diseases such as rabies, and helps to keep our food supply safe by testing food products for bacteria that may cause food-borne illness.

The ADRDL is one of fewer than 40 veterinary diagnostic laboratories accredited by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). In addition, the lab is an integral member of the National Animal Health Laboratory Network (NAHLN), a network of diagnostic laboratories across the US that help detect nationally significant animal diseases such as influenza and Foot & Mouth Disease. Expertise in detecting agents of food-borne illness is important in the ADRDL's role as a regional laboratory for the Food Emergency Response Network (FERN).