Seneca Valley Virus in Swine:
In 2015, the incidence of Seneca Valley Virus has increased and clinical signs in pigs can be observed. The clinical signs are often very similar to other vesicular foreign animal disease symptoms. Therefore, if vesicular lesions are observed, the State Veterinarian's office should be contacted as the first step in diagnosis Dr. Dustin Oedekoven, (firstname.lastname@example.org) Phone:605-773-3321, Fax: 605-773-5459) http://aib.sd.gov/ The South Dakota Animal Disease Research and Diagnostic Laboratory currently has a real-time PCR available for testing for this virus.
For more information on Seneca Valley Virus, please see the following web link from the Swine Health Information Center: http://www.swinehealth.org/emerging-disease-information/
Vesicular Stomatitis Virus
Vesicular Stomatitis in South Dakota - For testing horses for VSV at the laboratory:
- Contact the SD AIB (605) 773-3321
- Your veterinarian should collect BOTH:
- serum (for CF testing )
- swabs from the vesicular lesions (can be sent in TBTB media or viral transport media) for PCR testing.
- Send to the SD Animal Disease Research and Diagnostic Laboratory (SD ADRDL), (605) 688-5171, with the submission form (see http://www.sdstate.edu/vs/adrdl) and request VSV CF and VSV PCR
Canine Influenza Testing – SDSU ADRDL
NOTE: Timing of collection is critical to viral detection as canine influenza virus is only shed 2–4 days after initial clinical signs. Virus is not likely to be present in samples taken later in the course of disease, even if the dog is still showing clinical signs.
Swab: Polyester, Dacron, or rayon swab with a plastic shaft (BD BLL sterile culture swabs are OK)
- Collect both nasal and deep pharyngeal swabs should be collected.
- Nasal swabs: insert swab into nares.
- Pharyngeal swabs: swab the back of the throat,near the end of the soft palate.
- Place swab back into tube and add several drops of saline to keep the tube moist.
- If you have parafilm, cover the tube with it to seal it.
- Keep the samples refrigerated until they can be mailed (preferably the day of sampling).
- Mail with ice packs and mail overnight.
Testing is performed daily at a cost of $40/sample. The test is a real-time PCR procedure that detects Influenza A. If subtyping to characterize the virus as the canine H3N2 strain is desired, we can forward the sample on for further testing.
To submit, go to http://www.sdstate.edu/vs/adrdl/index.cfm click on "submission and other forms" on the left side of the page to find the "all species health form." Write in "canine influenza testing" and include the form with the sample.
Prompt and Accurate Veterinary Diagnostic Services
The South Dakota Animal Disease Research and Diagnostic Laboratory (ADRDL) has served the citizens of the state and region with timely and accurate veterinary diagnostic services since 1887. A dedicated and experienced staff performs a full range of diagnostic testing services that arm veterinarians and health officials with the information they need to protect and improve animal and therefore, human health.
The staff of the ADRDL are nationally recognized for their skill in diagnosing key diseases of cattle, pigs, and other livestock. Additionally, the ADRDL supports veterinarians and caretakers of horses and companion animals, plays a vital role in identifying zoonotic diseases such as rabies, and helps to keep our food supply safe by testing food products for bacteria that may cause food-borne illness.
The ADRDL is one of fewer than 40 veterinary diagnostic laboratories accredited by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). In addition, the lab is an integral member of the National Animal Health Laboratory Network (NAHLN), a network of diagnostic laboratories across the US that help detect nationally significant animal diseases such as influenza and Foot & Mouth Disease. Expertise in detecting agents of food-borne illness is important in the ADRDL's role as a regional laboratory for the Food Emergency Response Network (FERN).
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