Tamer Sharafeldin

Education

Bachelor of Veterinary Medical Sciences (BVSc), Zagazig University, Egypt 2004 (Major: Veterinary Medicine)
Masters of Veterinary Medical Sciences (MVSc), Zagazig University, Egypt 2008 (Major: Veterinary Pathology)
Doctor of Philosophy (Ph.D.), University of Minnesota, 2014 (Major: Veterinary Medicine-Veterinary Pathology)
Diplomate of the American College of Veterinary Pathologists

Academic Interests

Veterinary Diagnostic Pathology
Avian Pathology/Immunology/Vaccinology
Avian Reovirus (Turkey reoviruses)
Avian Infectious Bronchitis Virus (IBV)

Academic Responsibilities

Regular Diagnostic Pathology
Research
Teaching

Committee Activities

SDSU Faculty Senate
PPVM Curriculum Committee

Extension Responsibilities

Turkey industry
Game birds
Backyard small poultry flocks

Awards and Honors

2014 The graduate student travel award to travel to the Western Poultry Disease Conference, Puerto Vallarta, Mexico.

2014 The graduate student research award, North Central Avian Disease Conference, Saint Paul, MN.

2009 Partnership and Ownership (ParOwn) Award for pathology training at the University of Minnesota Veterinary Diagnostic Laboratory.

Grants

1- Deploying Baculovirus Vector in Protection Against Poultry Viral Diseases with Validation of Supportive Serologic Assays,
SD BOR competitive research grant ($88.349). Principal Investigator
2- Development and evaluation of a novel viral vectored vaccine to protect pigs against porcine reproductive and respiratory syndrome virus-
Haarberg 3D research center ($99,597). Co-Investigator.
3- Control strategies for emerging variants of turkey reovirus-USDA-NIFA AFRI ($299,907). Co-Investigator.
4- Surveillance, Rapid Detection, and Characterization of SARS-CoV-2 in Farmed and Wild Animals
USDA-APHIS ARS ($2,156,152). Co-Investigator.
5- Development of a serologic assay and novel vaccine approach to mitigate turkey arthritis reovirus
Minnesota Turkey Research & Promotion Council
$70,000 (2021-2023)
Principal Investigator
6- Turkey Reovirus solutions
South Dakota Poultry Industry Council (SDPIC)
$15,000 (2021)
7- Pathogenicity and Genetic Profile of Turkey Hepatitis Reovirus (THRV).
United States Poultry and Egg (USPE)
$64,369 (2020-2021)
Co-Investigator
8- Development and Evaluation of vaccination strategies against turkey arthritis/tenosynovitis reovirus.
United States Poultry and Egg (USPE)
$122,804 (2018-2020)
Principal Investigator
9- Immunologic correlates of avian reovirus: developing a diagnostic and serotyping ELISA for turkey reovirus.
Pennsylvania Department of Agriculture (PDA)
$25,000 (2019)
Principal Investigator
10- Characterization of strains of infectious bronchitis virus in Pennsylvania: Survey and molecular diagnostic technology development.
Pennsylvania poultry industry research Check-Off program
$20,000 (2018)
Co- Principal Investigator
11- Studies on effects of newly emerging avian reovirus variants on egg-laying hens and efficacy of non-metallic disinfection on reovirus.
Egg Industry Council (EIC)
$89,917 (2018-2020)
Co- Principal Investigator
12- Pathogenesis of newly emergent turkey arthritis reovirus in turkeys.
Rapid Agriculture Response Funding (RARF), University of Minnesota
$250,000 (2012-2015)
Graduate student collaborator

Professional Memberships

American College of Veterinary Pathologists.
American Association of Avian Pathologists.

Area(s) of Research

- Masters of Veterinary Sciences
▪ Experimental study of the interaction between a vaccine strain and a very virulent strain of infectious bursal disease virus.
▪ Evaluation of the interaction using histopathologic lesions scoring of bursal lesions and RT-PCR/ RFLP.

- PhD in Veterinary Medicine
▪ Studied the short- and long-term pathogenicity of Turkey arthritis reovirus (TARV) in turkeys and in chickens, virus pathogenesis and cytokine immune
responses in TARV-infected turkeys.

- Further research experiences:
▪ Surveillance and experimental studies to investigate the role of enteric viruses in poult enteritis complex in Minnesota.
▪ Diagnostic research on avian influenza virus, Newcastle disease virus and oncogenic viruses in poultry production in Egypt.
▪ Experience with sequencing technology, Sanger sequencing, next generation sequencing (library prep, DNA, RNA seq, data analysis).
▪ Vaccine research (Killed, live and recombinant vaccine development).
▪ Diagnostic assay development (Molecular and serologic assays).
▪ Surveillance to study the prevalence of different infectious bronchitis virus genotypes in Pennsylvania.
▪ Vaccine research for avian reovirus in the US.

Publications
1- Kumar R, Sharafeldin TA, Goyal SM, Mor SK, Porter RE, Infection and transmission dynamics of Turkey arthritis reovirus in different age Turkeys, Microbial
Pathogenesis, Volume 173, Part A, 2022,
2- Kumar R, Sharafeldin TA, Sobhy NM, Goyal SM, Porter RE & Mor SK (2022) Comparative pathogenesis of turkey reoviruses, Avian Pathology, 51:5, 435-444, DOI:
10.1080/03079457.2022.2079474
3- Temeeyasen G, Sharafeldin TA, Lin C, Hause BM. Spillover of Canine Parvovirus Type 2 to Pigs, South Dakota, USA, 2020. Emerg Infect Dis. 2022;28(2):471-473.
https://doi.org/10.3201/eid2802.211681
4- Kumar P, Sharafeldin TA, Kumar R, Huang Q, Liang Y, Goyal SM, Porter RE, Ly H, Mor SK. Development of a Recombinant Pichinde Virus-Vectored Vaccine against
Turkey Arthritis Reovirus and Its Immunological Response Characterization in Vaccinated Animals. Pathogens. 2021 Feb 13;10(2):197. doi:
10.3390/pathogens10020197. PMID: 33668435; PMCID: PMC7918942.
5- Aboubakr HA, Sharafeldin TA, Goyal SM. Stability of SARS-CoV-2 and other coronaviruses in the environment and on common touch surfaces and the influence of
climatic conditions: A review. Transbound Emerg Dis. 2020; 00: 1– 17. https://doi.org/10.1111/tbed.13707
6- Sharafeldin TA, Mor SK, Goyal SM, Porter RE. Altered Biomechanical Properties of Gastrocnemius Tendons of Turkeys Infected with Turkey Arthritis Reovirus. Vet Med
Int. 2016. DOI: 10.1155/2016/7829138
7- Sharafeldin TA, Mor SK, Abdel-Glil MY, Goyal SM. Prevalence of turkey parvovirus in Minnesota turkeys. Poult Sci. 2016 doi: 10.3382/ps/pew283.
8- Sharafeldin TA, Mor SK, Sobhy NM, Xing Z, Reed K, Goyal SM, Porter RE. A newly emergent turkey arthritis reovirus shows dominant enteric tropism and induces
significantly elevated innate antiviral and T helper-1 cytokine responses. PlosOne. 2015 Dec 11;10(12): e0144085.
9- Ali A, Elmowalid G, Abdel-Glil M, Sharafeldin T, Abdallah F, Mansour S, Nagy A, Ahmed B, Abdelmoneim, M. Etiology and pathology of epidemic outbreaks of avian
influenza H5N1 infection in Egyptian chicken farms. Pol J Vet Sci. 2015;18(4):779-86.
10- Sharafeldin TA, Mor SK, Bekele AZ, Verma H, Ismagilova L, Goyal SM, Porter RE. The pathogenicity of a newly emergent turkey arthritis reovirus in chickens. Poult
Sci. 2015 Oct;94(10):2369-74.
11- Sharafeldin TA, Mor SK, Bekele AZ, Verma H, Noll SL, Goyal SM, Porter RE. Experimentally induced lameness in turkeys inoculated with a newly emergent turkey
reovirus. Vet Res. 2015 Feb 24;46(1):11.
12- Mor SK, Bekele AZ, Sharafeldin TA, Porter RE, Goyal SM. Efficacy of Five Commonly Used Disinfectants Against Turkey Arthritis Reovirus. Avian Dis 03/2015;
59(1):71-73.
13- Mor SK, Verma H, Sharafeldin TA, Porter RE, Ziegler AF, Noll SL, Goyal SM. Survival of turkey arthritis reovirus in poultry litter and drinking water. Poult Sci. 2015
Apr;94(4):639-42.
14- Mor SK, Marthaler D, Verma H, Sharafeldin TA, Jindal N, Porter RE, Goyal SM. Phylogenetic analysis, genomic diversity and classification of M class gene segments of
turkey reoviruses. Vet Microbiol. 2015 Mar 23;176(1-2):70-82.
15- Mor SK, Verma H, Bekele AZ, Sharafeldin TA, Porter RE, Goyal SM. One-step real-time reverse transcription-PCR for the detection of turkey reoviruses. Avian Dis.
2014 Sep;58(3):404-7.
16- Mor SK, Sharafeldin TA, Porter RE, Goyal SM. Molecular characterization of L class genome segments of a newly isolated turkey arthritis reovirus. Infect Genet Evol.
2014 Oct;27:193-201.
17- Mor SK, Verma H, Sharafeldin TA, Porter RE, Jindal N, Ziegler A, Goyal SM. Characterization of S class gene segments of a newly isolated turkey arthritis reovirus.
Virology. 2014 Sep;464-465:33-44.
18- Sharafeldin TA, Mor SK, Bekele AZ, Verma H, Goyal SM, Porter RE. The role of avian reoviruses in turkey tenosynovitis/arthritis. Avian Pathol. 2014;43(4):371-8.
19- Abdel-Glil MY, Mor SK, Sharafeldin TA, Porter RE, Goyal SM. Detection and characterization of Newcastle disease virus in formalin-fixed, paraffin-embedded tissues
from commercial broilers in Egypt. Avian Dis. 2014 Mar;58(1):118-23.
20- Mor SK, Sharafeldin TA, Abin M, Kromm M, Porter RE, Goyal SM, Patnayak DP. The occurrence of enteric viruses in Light Turkey Syndrome. Avian Pathol.
2013;42(5):497-501.
21- Mor SK, Sharafeldin TA, Porter RE, Ziegler A, Patnayak DP, Goyal SM. Isolation and characterization of a turkey arthritis reovirus. Avian Dis. 2013 Mar;57(1):97-103.

Applications of Research

Development of Diagnostic Assays-Turkey Reovirus ELISA and rRT-PCR.
Vaccines Against Turkey Reoviruses.